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Training Methodologies.
I.
CHROMATOGRAPHIC TECHNIQUES:
a. Paper Chromatography
b. Thin layer Chromatography (TLC)
c. Column Chromatography
i. Affinity Chromatography
ii. Ion Exchange Chromatography
iii. Molecular (Gel ) exclusion
Chromatography
II. ELECTROPHORESIS TECHNIQUES:
a. SDS - PAGE.
b. Agarose Gel Electrophoresis.
c. Serum electrophoresis.
d. Immuno electrophoresis.
e. Counter current immuno electrophoresis.
f. Rocket immuno electrophoresis.
g. Pulsed field gel electrophoresis (FIGE)
h. Single cell gel electrophoresis
III.
Precipitation and Agglutination Techniques:
a. Raising antiserum in rabbits with adjuvant
b. Manicini's single diffusion techniques
c. Ouchterlony's double diffusion techniques
d. Radial immuno diffusion techniques
e. Plate ELISA
f. Dipstick Dot - ELISA
g. Haemagglutination Test (HA) Viral Detection.
h. Haemagglutination inhibition test (HI). Viral Anti body Detection.
i. Macroscopic slide agglutination test (MSAT).
j. Microscopic agglutination test (MAT) for Leptospira.
IV. ELECTROBLOTTING TECHNIQUES:
a. Western / Immunoblotting technique
b. Southern blotting technique
c. Northern blotting technique
V. MOLECULAR BIOLOGICAL TECHNIQUES:
a. Genomic DNA extraction and demonstration from
i. Plant source
ii. Animal Source
iii. Microbial source
b. Plasmid DNA isolation and demonstration.
c. Restriction digestion (RFLP)
d. Ligation
e. Transformation
f. Phage titration
g. Generalized and Specialized Transduction
h. Interrupted and uninterrupted Conjugation
i. Polymerase chain reaction (PCR) and Gel documentation
j. Rapid amplification of polymorphic DNA (RAPD)
k.
Protoplasmic fusion.
l.
Nucleic acid probe construction.
m.
Southern Hybridization.
n.
Northern Hybridization.
o.
Site directed mutagenesis.
VI. TISSUE CULTURE TECHNIQUES:
I. Plant
a. Micropropagation
b. Cell / Protoplast culture (callus culture)
c. Organ culture
d. Haploid & double haploid cultures.
II.
Animal
a. Primary cell culture (Chick embryo fibroblast culture)
III. Cell line maintenance and sub culturing e.g. VEERO, BHK21 & MDCK.
IV. Animal virus isolation, purification and the antigen
estimation techniques.
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